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在用ＭＴＴ测定细胞存活率以研究人表皮癌细胞ＫＢ－３－１ 及其抗阿霉素细胞株ＫＢ－Ａ－１对化疗药物阿霉素、长春花碱和秋水仙碱的多药抗性时，发现培养基中血清浓度的变化对ＭＴＴ法测定结果有影响。吸光值在血清浓度为ｗ ＝ ０．０２ 时最高，ｗ ＝ ０．３０ 时最低，ｗ ＝ ０．０５～０．２０ 之间波动不大。而不加血清使甲酉尤溶解困难而产生误差。培养基中的血清（常为ｗ ＝ ０．１０）不影响ＭＴＴ法的结果，在完全培养基中用ＭＴＴ法测定了两株细胞的抗药性。ＫＢ－Ａ－１ 对阿霉素、长春花碱和秋水仙碱的相对抗药性分别为ＫＢ－３－１ 的１７６、４５ 和１３７ 倍。ＭＴＴ法可简便地用于抗癌药物的筛选。
The cell viability was measured by MTT to study the multidrug resistance of human epidermal cancer cell line KB-3-1 and its anti-adriamycin cell line KB-A-1 against chemotherapeutic drugs doxorubicin, vinblastine and colchicine. At the time, it was found that changes in the serum concentration in the medium had an effect on the results of the MTT assay. Absorbance values were highest when the serum concentration was w = 0.02, lowest when w = 0.30, and fluctuating little between w = 0.05 and 0.20. The addition of serum does not make it difficult to dissolve the hyperthyroidism. Serum in the medium (usually w = 0.10) did not affect the results of the MTT assay. Both cells were tested for drug resistance in the complete medium using the MTT assay. The relative drug resistance of KB-A-1 to adriamycin, vinblastine, and colchicine was 176, 45, and 137 times that of KB-3-1, respectively. The MTT method can be simply used for the screening of anticancer drugs.