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目的本实验旨在了解大黄提取液对牙釉质的抗酸性的影响。方法取制备好40枚磨牙釉质标本,并分为5小组:(1)1mg/ml大黄提取液组;(1)2mg/ml大黄提取液组;(3)4mg/ml大黄提取液组;(4)2%氟化钠(NaF)组;(5)去离子水组。在每组8枚釉质标本开窗处分别应用上述5种溶液进行预处理7d,4m-m/次,3次/d,处理间隙置于37℃人工唾液中保存,然后将釉质标本10.1mol/L乳酸脱矿液中在37℃恒温水浴葙7d。采用扫描电镜观察处理后每小组的两个釉质标本表面形态的变化。结果扫描电镜观察5组釉质表面都有不同程度的溶解,经3组大黄提取液处理的釉质表面和去离子水组比表现出较明显的轻微程度的溶解,其他4组的溶解程度没有表现出明显差异。结论3组大黄提取液都具有抑制釉质脱矿作用;1mg/ml大黄提取液为抑制釉质脱矿的起始有效浓度。
Objective This experiment aims to understand the effect of rhubarb extract on enamel’s acid resistance. Methods A total of 40 molar enamel specimens were prepared and divided into 5 groups: (1) 1 mg/ml rhubarb extract group; (1) 2 mg/ml rhubarb extract group; (3) 4 mg/ml rhubarb extract group; 4) 2% sodium fluoride (NaF) group; (5) deionized water group. Each group of 8 enamel samples were treated with the above 5 solutions for 7 days, 4m-m/time, 3 times/day, and the treatment gap was preserved in artificial saliva at 37°C. Then the enamel specimen was 10.1 mol/mol. The L-lactate demineralization solution was kept in a thermostatic bath at 37°C for 7 days. Scanning electron microscopy was used to observe the surface morphological changes of the two enamel specimens after treatment. RESULTS: Scanning electron microscopy showed that the enamel surfaces of the 5 groups had different degrees of dissolution. The enamel surface treated with the rhubarb extracts of the 3 groups and the deionized water group exhibited a more obvious degree of dissolution than the other groups. The dissolution degree of the other 4 groups was not shown. Significant differences. Conclusion The rhubarb extracts from all three groups have the effect of inhibiting enamel demineralization; 1 mg/ml rhubarb extract is the initial effective concentration to inhibit enamel demineralization.