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目的观察盐酸吉西他滨对慢性粒细胞白血病(慢粒)急变期细胞增殖凋亡的影响,探讨盐酸吉西他滨用于慢性粒细胞白血病急变期治疗的可行性。方法采用水溶性四唑盐比色法检测盐酸吉西他滨对慢粒急变期K562细胞增殖的影响,采用流式细胞术AnnexinV/FITC法检测盐酸吉西他滨对慢粒急变期K562细胞凋亡的影响。结果 0.1 mg/L、1.0 mg/L、10 mg/L盐酸吉西他滨分别作用K562细胞12 h、24 h、48 h、72 h后,K562细胞增殖受到抑制,抑制作用随浓度时间增加而增强,但48 h和72 h相比差异无统计学意义。吉西他滨组对K562细胞的抑制作用明显强于同浓度同时间的阿糖胞苷组,两者比较差异有统计学意义(P<0.01)。流式细胞术AnnexinV/FITC法检测结果显示10 mg/L吉西他滨作用于K562细胞48 h凋亡率为(20.56±0.02)%,明显高于阿糖胞苷组(4.68±0.01)%(P<0.01)和空白对照组(1.92±0.01)%(P<0.01)。结论盐酸吉西他滨对慢粒急变期K562细胞有一定的增殖抑制及促凋亡作用,且具有剂量和时间依赖性,有望联合其他药物应用于慢粒急变期的治疗。
Objective To investigate the effect of gemcitabine hydrochloride on the proliferation and apoptosis of chronic myeloid leukemia (CML) in acute phase and to explore the feasibility of gemcitabine hydrochloride for the treatment of chronic myeloid leukemia. Methods The effect of gemcitabine hydrochloride on the proliferation of K562 cells was detected by water-soluble tetrazolium salt colorimetric assay. The effect of gemcitabine hydrochloride on the apoptosis of K562 cells was detected by flow cytometry AnnexinV / FITC. Results K562 cells were inhibited by 0.1 mg / L, 1.0 mg / L and 10 mg / L gemcitabine hydrochloride for 12 h, 24 h, 48 h and 72 h, respectively. The inhibitory effect was enhanced with increasing concentration time, 48 h and 72 h compared with no significant difference. The inhibitory effect of gemcitabine on K562 cells was significantly stronger than that of cytarabine at the same concentration and at the same time, the difference was statistically significant (P <0.01). Flow cytometry AnnexinV / FITC assay showed that the apoptosis rate of K562 cells treated with 10 mg / L gemcitabine for 48 h was (20.56 ± 0.02)%, significantly higher than that of cytarabine (4.68 ± 0.01)% (P < 0.01) and blank control group (1.92 ± 0.01)% (P <0.01). Conclusion Gemcitabine hydrochloride can inhibit proliferation and induce apoptosis of K562 cells in a dose-and time-dependent manner. It is expected that gemcitabine may be used in combination with other drugs in the treatment of chronic myolectomy.