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目的:探讨鼻咽癌组织中EB病毒(EBV)定量检测的意义。方法:应用荧光定量PCR(real-timePCR)技术定量检测30例鼻咽低分化鳞癌患者癌组织、癌旁组织及15例正常健康人鼻咽黏膜组织中的EBV拷贝数,并比较三者检测结果。结果:正常人鼻咽黏膜EBV检出率为73.3%(11/15),平均拷贝数为6.5×102μg-1;鼻咽癌和癌旁组织EBV检出率为100%(30/30)和76.7%(23/30),平均拷贝数分别为6.7×105μg-1DNA和1.3×105μg-1DNA。正常人鼻咽黏膜、鼻咽癌患者癌旁组织感染EBV的概率差异无统计学意义(P>0.05);鼻咽癌组织、癌旁和正常黏膜组织3者之间,鼻咽癌与癌旁组织之间,癌旁和正常黏膜组织之间EBV拷贝数差异有统计学意义(P<0.01)。结论:鼻咽癌与癌旁组织感染EBV的数量明显高于正常鼻咽黏膜,EBV感染量是鼻咽癌致病的重要因素,定量检测鼻咽癌组织中EB病毒有助于临床判断病情。
Objective: To investigate the significance of quantitative detection of Epstein-Barr virus (EBV) in nasopharyngeal carcinoma. Methods: Quantitative real-time PCR (RT-PCR) was used to detect EBV copy number in nasopharyngeal mucosa of 30 cases of poorly differentiated squamous cell carcinoma of nasopharyngeal carcinoma tissues, adjacent normal tissues and 15 healthy controls. result. Results: The detection rate of EBV in nasopharyngeal mucosa was 73.3% (11/15) and the average copy number was 6.5 × 102μg-1. The detection rate of EBV in nasopharyngeal carcinoma and paracancer tissues was 100% (30/30) and 76.7% (23/30), the average copy number was 6.7 × 105μg-1DNA and 1.3 × 105μg-1DNA, respectively. There was no significant difference in the probability of EBV infection among normal nasopharyngeal mucosa and NPC tissues (P> 0.05); between nasopharyngeal carcinoma tissues, adjacent normal mucosa tissues and normal mucosa tissues, There were significant differences in EBV copy number between tissues, adjacent normal mucosa and normal mucosa (P <0.01). Conclusion: The number of EBV infection in nasopharyngeal carcinoma and adjacent non-cancerous tissues is significantly higher than that in normal nasopharyngeal mucosa. The EBV infection is an important factor in the pathogenesis of nasopharyngeal carcinoma. Quantitative detection of Epstein-Barr virus in nasopharyngeal carcinoma is helpful for clinical judgment.