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目的：肺癌是高恶性和高死亡率的肿瘤之一，作者力图从生物力学角度揭示肺癌细胞侵润转移的某些生物学特性。材料与方法：本文结合超微结构观察,采用微管吸吮技术定量测定体外培养的低转移人肺腺癌(PAa)细胞和高转移人肺巨细胞癌(PG)细胞在长春新碱(VCR)作用下的粘弹特性。结果：肺癌细胞粘弹性,在未使用VCR前为PAa细胞大于PG细胞；随VCR的使用和剂量增加,其在PAa细胞呈浓度依赖性下降,在PG细胞则表现为低VCR浓度(0．10 μg／ml)时降低而后以弹性系数K1,K2为主的增高。超微结构观察见PAa细胞微管、微丝稀疏,VCR作用后进一步减少；PG细胞微管、微丝减缺,VCR作用后分裂期形态表现多见。结论：细胞内微管、微丝等细胞骨架成份的改变与癌细胞运动迁徙能力有关,VCR等细胞骨架干扰剂的抗肿瘤作用可能主要通过调控癌细胞的增殖分化信息来影响癌细胞生物学特性及侵润转移能力。
OBJECTIVE: Lung cancer is one of high-grade and high-mortality tumors. The authors sought to reveal some of the biological characteristics of lung cancer cell invasion and metastasis from a biomechanical perspective. MATERIALS AND METHODS: In this paper, ultrastructure observation was used to quantitatively measure low metastatic human lung adenocarcinoma (PAa) cells and high metastatic human lung giant cell carcinoma (PG) cells cultured in vitro using the micropipette suction technique in vincristine (VCR). The role of viscoelastic properties. RESULTS: The viscoelasticity of lung cancer cells was larger than that of PG cells before VCR was used. With the use of VCR and increasing dose, it decreased in a concentration-dependent manner in PAa cells and showed a low VCR concentration in PG cells (0.10). Μg/ml) decreased and then the elastic coefficient K1, K2 increased. Ultrastructural observation revealed that PAa cells were microtubules and microfilaments were sparse. After VCR treatment, they were further decreased. Microtubules and microfilaments of PG cells were reduced. VCR showed more morphological appearance at the cleavage stage. Conclusion: The changes of intracellular microtubules, microfilaments and other cytoskeletal components are related to the ability of cancer cells to migrate. The antitumor effect of cytoskeleton interfering agents such as VCR may affect the biological characteristics of cancer cells mainly through regulating the proliferation and differentiation of cancer cells. Invasion and transfer ability.