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副溶血性弧菌 (Ｖｉｂｒｉｏｐａｒａｈａｅｍｏｌｙｔｉ ｃｕｓ,Ｖｐ)是一种能引起食物中毒和旅游者腹泻的嗜盐性细菌。直接溶血毒素(ｔｈｅｒｍｏｓｔａｂｌｅｄｉｒｅｃｔｈｅｍｏｌｙｓｉｎ,ＴＤＨ)是其主要的致病因子。本研究通过构建ｔｄｈ基因原核表达载体 ,并实现表达
Vibrioparahaemolyti cus (Vp) is a halophilic bacterium that causes food poisoning and travelers’ diarrhea. Direct hemolytic toxin (thermostabledirecthemolysin, TDH) is its main virulence factor. In this study, tdh gene prokaryotic expression vector was constructed and expressed