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目的比较α1肾上腺素受体(α1-AR)激动剂苯肾上腺素对正常及链脲霉素(STZ)诱导的糖尿病大鼠室性心律失常的差异作用,及对两组大鼠左心室肌细胞早期后除极(EAD)的差异作用;并分析两组大鼠左心室α1-AR各亚型的差异表达,解析其可能的物质基础。方法采用离体心脏心电图及电流钳法,分析α1-AR激动剂(苯肾上腺素,PE)诱导正常和糖尿病大鼠离体心脏心律失常发生率及左心室肌细胞EAD发生率的差异作用。采用Westernblot法,比较两组大鼠左心室α1-AR各亚型的蛋白表达。结果与正常大鼠相比,PE(100μmol.L-1)诱导糖尿病大鼠离体心脏产生室性心律失常的机率明显增加,PE(1000μmol.L-1)诱导左心室肌细胞产生EAD的机率明显增加;α1A-AR在糖尿病大鼠左心室的表达明显增加。结论α1-AR激动剂使糖尿病大鼠左心室肌细胞产生高机率的EAD是其诱导高机率室性心律失常的基础。而糖尿病大鼠左心室α1A-AR表达明显增加是其物质基础。
Objective To compare the different effects of phenylephrine, an α1-adrenoceptor agonist, on ventricular arrhythmia induced by STZ in normal and streptozotocin (STZ) -induced diabetic rats and to compare the effects of phenylephrine on left ventricular myocytes Early post-depolarization (EAD) differences in the role; and analysis of the two groups of rat left ventricular α1-AR subtypes of the differential expression analysis of its possible material basis. Methods The effects of α1-AR agonist (phenylephrine, PE) on the incidence of isolated cardiac arrhythmia and the incidence of left ventricular myocytes EAD in normal and diabetic rats were analyzed by using isolated heart electrocardiogram and current clamp method. Western blot was used to compare the protein expression of α1-AR in the left ventricle in both groups. Results Compared with normal rats, PE (100 μmol·L-1) induced a significant increase of ventricular arrhythmia in isolated rat hearts and PE (1000 μmol·L-1) induced the incidence of EAD in left ventricular myocytes Significantly increased; α1A-AR in diabetic rats significantly increased left ventricular expression. Conclusion The α1-AR agonist can induce the high incidence of EAD in left ventricular myocytes of diabetic rats. Diabetic rats left ventricular α1A-AR expression was significantly increased its material basis.