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目的探讨高原寒区平、战时枪弹伤对机体健康肌肉组织酶活性和物质消耗的影响。方法将24头实验猪随机分为高原战时致伤组(GZ)、高原平时致伤组(GP)和平原平时致伤组(PP),每组8头,分别于伤前、伤后不同时相点取背部健康肌肉组织匀浆后定量测定Na+-K+-ATP酶、乳酸脱氢酶(lactate dehydrogenase,LDH)、磷酸肌酸激酶(creatine kinase,CK)、肌糖元(muscle glycogen,MG)、乳酸(lactic acid,LA)、游离无机磷(Pi3+)的含量。结果①伤后2hPP组、GP组和GZ组LDH的活性分别是伤前值的3.73倍、2.76倍和2.75倍,PP组伤后2d开始降低、而GZ、GP两组从伤后2h开始逐渐上升,到5d时与平原组接近。②三组动物伤后CK均逐渐升高并于伤后12h到顶峰,PP组、GP组和GZ组分别为伤前的2.07倍、1.77倍和1.70倍。③三组动物伤后ATP酶活性均显著升高,并于伤后6h达最高点,PP组、GP组和GZ组分别为伤前的2.29倍、1.75倍和1.62倍,此后逐渐下降。④三组伤后肌糖元变化规律相似,在伤后2h形成一个显著的低点,PP组、GP组和GZ组分别为伤前的81.5%、75.3%和73.8%,此后逐渐回升。⑤GZ组伤后2h时乳酸达峰值,为GP组的1.58倍,此后有逐渐下降的趋势。⑥三组动物伤后健康肌肉组织中的游离无机磷逐渐减少,伤后2d～3d出现一个显著的低点,下降的幅度大小依次为高原战时组>高原平时组>平原平时组,高原战时组最低值为高原平时组最低值的84.2%,为伤前的39.0%。结论高原战时环境枪弹伤后机体组织酶活性储备能力降低,可代偿能力下降,长时间处于高耗能状态。
Objective To investigate the effects of bullet wounds on the activities of healthy muscle tissue enzymes and the material consumption in flat and wartime cold plateau areas. Methods Twenty-four experimental pigs were randomly divided into GZ group, GP group and GP group, with 8 rats in each group. The animals were randomly divided into two groups: pre-injury and post-injury In the meantime, Na + -K + -ATPase, lactate dehydrogenase (LDH), creatine kinase (CK), muscle glycogen (MG) ), Lactic acid (LA), free inorganic phosphorus (Pi3 +) content. Results ① The activities of LDH in PP group, GP group and GZ group were 3.73 folds, 2.76 folds and 2.75 folds, respectively, at 2 h after injury, and PPH group began to decrease 2 d after injury, while the GZ and GP groups began to gradually decrease from 2 h after injury Rose to close to the plains group by 5 days. ② CK in the three groups of animals increased gradually and peaked at 12h after injury. The levels of PP, GP and GZ were 2.07, 1.77 and 1.70 times of pre-injury, respectively. ③ The activity of ATPase in three groups of animals increased significantly and reached the highest point at 6h after injury. The levels in PP, GP and GZ groups were 2.29, 1.75 and 1.62 times before injury, respectively, and then decreased gradually. ④ The changes of muscular glycogen in the three groups were similar, forming a significant low point at 2h after injury. The PP group, GP group and GZ group were 81.5%, 75.3% and 73.8% of the pre-injury, respectively. ⑤ GZ group 2h after lactic acid peak, 1.58 times that of GP group, then gradually declining trend. ⑥The free inorganic phosphorus in healthy muscle tissue of the three groups of animals decreased gradually after injury, with a significant decrease from 2d to 3d after injury, and the magnitude of decrease was in the order of Wushu group> normal group> plain group, plateau war The lowest value of the hourly group was 84.2% of the lowest value of the daytime group and 39.0% of that before the injury. Conclusion After the wounded in the wartime, the reserve of tissue enzyme activity reserves decreased, the compensatory ability decreased, and the energy consumption was prolonged.