目前已有许多寻找抗肿瘤活性物质的方法,包括体内的各种动物肿瘤试验和体外的细胞培养法。本文着重讨论对细胞周期特异性作用的微生物发酵液的体外筛选方法。发酵液中虽含有许多活性物质,但活性浓度往往极低。在此情况下,应用体外筛选法最为有利。因为(1)以此可能找到有特异性作用的物质;(2)操作及所需样品比体内试验经济;(3)为区别新的物质和进一步化学或 There are many methods for finding antitumor active substances, including various animal tumor tests in vivo and cell culture methods in vitro. This article focuses on in vitro screening methods for cell cycle-specific microbial fermentation broth. Although the fermentation broth contains many active substances, the active concentration is often extremely low. In this case, the application of in vitro screening is the most advantageous. Because (1) it is possible to find substances with specific effects; (2) operations and required samples are more economical than in vivo tests; (3) to distinguish between new substances and further chemicals or