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我国目前动物实验主要用小鼠进行。然而经典的Ｓｅｇｌｅｎ灌流肝细胞分离需采用门静脉插管灌流较难用于幼鼠 ,特别是幼小鼠。我们对Ｓｅｇｌｅｎ灌流法做了改进 ,将生后 2ｄ的 6 15幼小鼠肝细胞培养成功 ,现报告如下。1 材料和方法动物 :出生 2ｄ 6 15幼鼠。试剂 :Ｄ -Ｈａｎ
Animal experiments in our country are mainly conducted with mice. However, classical Seglen perfused hepatocytes require portal perfusion to be more difficult to apply to young rats, especially young mice. Seglen perfusion method we have done an improvement, 2 days after birth 6 15 young mouse hepatocyte culture is successful, are as follows. 1 Materials and Methods Animals: 2d 6 15 born young rats. Reagents: D -Han