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目的 :观察组织块原代培养法是否可用于涎腺肌上皮细胞发育分化的研究 ,并探讨肌上皮细胞的组织发生。方法 :采用组织块原代培养法 ,通过相差显微镜、透射电镜及免疫组化染色等检测手段 ,对不同时期大鼠颌下腺肌上皮细胞进行研究。结果 :肌上皮样细胞及含有分泌颗粒的分泌细胞见于体外培养第 3d。肌微丝及Actin阳性细胞分别最早出现于培养第 6d和 7d。此结果与体内研究结果相一致。结论 :肌上皮细胞可能来源于上皮干细胞 ;体外组织块培养方法亦适用于细胞分化研究。
OBJECTIVE: To observe whether the primary culturing method of tissue blocks can be used to study the development and differentiation of myogenetic myoepithelial cells and to explore the histogenesis of myoepithelial cells. Methods: Primary culture of tissue was used to detect the submandibular gland myoepithelial cells in different periods by phase contrast microscopy, transmission electron microscopy and immunohistochemical staining. Results: Myoepithelial cells and secretory granules containing secretory cells were found in vitro in 3d. Muscle actin filaments and actin positive cells were first appeared in culture 6d and 7d. This result is consistent with in vivo studies. Conclusion: Myoepithelial cells may originate from epithelial stem cells. In vitro tissue culture methods are also suitable for cell differentiation research.