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目的:研究脾虚模型脑内一氧化氮(NOS)与乙酰胆碱酯酶(ACHE)表达变化及归脾汤的影响作用。方法:采用苦降泻下、饮食失节加劳倦过度法建立脾虚大鼠模型,免疫组化法检测下丘脑腹侧核、海马CA1区、前额叶皮层NOS与ACHE变化及归脾汤治疗的影响作用。结果:模型组NOS和ACHE免疫阳性反应物在下丘脑腹侧核、海马CA1区、前额叶皮层明显降低;治疗组上述脑区的NOS和ACHE免疫阳性反应物明显增加。结论:脾虚模型脑内NOS和ACHE表达有变化,提示归脾汤通过影响NOS与ACHE而调节学习记忆功能。
Objective: To study the changes of nitric oxide (NOS) and acetylcholinesterase (ACHE) expression in brain of spleen deficiency model and the effect of Guipi decoction. Methods: The rat model of spleen deficiency was established by the method of bitter diarrhea, eating disorder, and fatigue. The changes of NOS and ACHE in hypothalamic ventral nucleus, hippocampal CA1 region, prefrontal cortex and the effect of Guipi Decoction were detected by immunohistochemistry. . RESULTS: The NOS and ACHE immunoreactive substances in the model group were significantly lower in the hypothalamic ventral nucleus, hippocampal CA1 region, and prefrontal cortex. The NOS and ACHE immunoreactive substances in the brain regions of the treated group were significantly increased. Conclusion: There is a change in the expression of NOS and ACHE in the brain of spleen deficiency model, suggesting that Guipi decoction regulates learning and memory function through affecting NOS and ACHE.