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以茶树幼苗为材料,以HPLC、qRT-PCR为分析手段,分析了茶树幼苗发育过程中根茎叶的儿茶素含量变化及相关合成基因的表达差异。结果显示,在发育过程中,不同器官中儿茶素总量呈现不同的变化规律,在幼苗发育25 d、45 d、75 d和90 d时,茶树叶片的儿茶素含量(干重)呈下降趋势,分别为79.99、57.69、49.29和33.26 mg.g-1;茎中(干重)的为14.40、11.89、8.54和9.29 mg.g-1;根中的含量(干重)很低,分别为1.03、0.60、0.82和1.62 mg.g-1;在发育25 d的茶树幼苗茎叶中6种儿茶素单体均可以检测到;根中缺乏酯型儿茶素,但在发育10 d的根中却可以检测到。在茶树幼苗不同器官中,酚类物质合成相关基因表达有较大的差异性。
Using tea seedlings as materials, the changes of catechins content and the expression of related synthetic genes were analyzed during the development of tea seedlings by HPLC and qRT-PCR. The results showed that the total amount of catechins in different organs exhibited different changes during development, and the catechin content (dry weight) of tea leaves at 25 d, 45 d, 75 d and 90 d after seedling development (Dry weights) were 14.40, 11.89, 8.54 and 9.29 mg.g-1 respectively. The content of root (dry weight) was very low, 1.03, 0.60, 0.82 and 1.62 mg.g-1, respectively. Six kinds of catechins could be detected in stems and leaves of tea seedlings after 25 days of development. There were no ester-type catechins in roots, d root can be detected. In different organs of tea tree seedlings, there are great differences in gene expression related to phenolic synthesis.