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为了测定不同产地柴胡中柴胡皂苷a、d的含量,试验采用超声提取和高效液相色谱含量测定的方法对10个不同产地样品中柴胡皂苷a、d的含量进行了研究。结果表明:在相同色谱条件下,柴胡皂苷a的平均回收率为90.71%,RSD为1.85%(n=6);柴胡皂苷d的平均回收率为92.67%,RSD为2.1%(n=6)。10个不同产地的样品中柴胡皂苷a、d的含量略有差别,其中辽宁葫芦岛、嵩县野生柴胡样品中柴胡皂苷a、d的总含量最高,而东北、嵩县田湖镇样品中柴胡皂苷a、d的总含量较其他产地略低,但各地区柴胡皂苷a、d的总含量均大于0.3%。说明不同地区柴胡药材中柴胡皂苷a和柴胡皂苷d的含量有一定差别。
In order to determine the content of saikosaponin a, d in different regions of Bupleurum, the content of saikosaponin a and d in 10 samples of different origins was studied by using ultrasonic extraction and high performance liquid chromatography. The results showed that the average recovery of saikosaponin a was 90.71% and the RSD was 1.85% (n = 6) under the same chromatographic conditions. The average recoveries of saikosaponin d was 92.67% and the RSD was 2.1% (n = 6). The content of saikosaponin a and d in 10 samples from different areas was slightly different, among which the total content of saikosaponin a and d in wild Bupleurum samples of Huludao and Songxian in Liaoning Province was the highest, while that of Tianhu Town in northeast and Songxian The total content of saikosaponins a and d in the samples was slightly lower than that in other areas, but the total contents of saikosaponin a and d in all regions were all greater than 0.3%. Indicating different regions Bupleurum medicinal saikosaponin saikosin a and saikosaponin d content of a certain difference.