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利用十二烷基磺酸钠聚丙烯酰胺凝胶电泳 (SDS PAGE)和等电聚焦双向电泳 (IEF 2D)对胡杨不同类型愈伤组织和愈伤组织分化不定芽过程中表达的蛋白进行了研究。发现 :不同类型愈伤组织中表达的蛋白存在着明显的差异。在光照和培养基中BA NAA值为 1时诱导产生的有极强器官发生能力的茎基愈伤组织 ,其蛋白组分明显地少于经过继代培养、器官发生能力明显下降的愈伤组织 ,表明了茎基愈伤组织本身分化程度低。实验获得了不同类型愈伤组织和愈伤组织分化不定芽过程中不同阶段所表达的特异蛋白 ,表现为经过黑暗和培养基中BA NAA值为 0 5的继代增殖培养 ,愈伤组织产生了特异的 2 4 5kDa和 5 8 6kDa的标记蛋白带 ,并且也表达了其器官发生时表达的 19kDa和 31kDa蛋白带。茎基愈伤组织在光照和BA NAA值为 0 5的条件下进行器官发生诱导并且随着愈伤组织形成分生细胞团块和不定芽原基 ,明显地表达了 2 0kDa和 5 5kDa蛋白带 ,在 2 0kDa蛋白带中含有pI为 5 5～ 6 5的特异蛋白。pI为 6 5～ 7 5的 4 3kDa为不定芽发生前期表达的特异蛋白。文中就愈伤组织器官发生能力与其表达蛋白的关系进行了讨论。
The proteins expressed in the process of adventitious bud differentiation from different types of callus and callus of Populus euphratica were studied by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and isoelectric focusing two-dimensional electrophoresis (IEF 2D) . It is found that there are obvious differences in the proteins expressed in different types of callus. The stem-based callus induced by BA NAA of 1 in the light and medium with strong organogenic ability was significantly less than that of the callus which had been subcultured and the organogenesis ability decreased significantly , Indicating that the stem callus itself has a low degree of differentiation. Experiments obtained different types of callus and callus differentiation adventitious buds during the expression of different stages of the specific protein showed that after dark and medium BA NAA value of 0 5 of the subculture proliferation callus generated Specific 24kDa and 586kDa marker bands and also expressed 19kDa and 31kDa protein bands expressed at the time of their organogenesis. Stem-based callus was organically induced under light and BA NAA values of 0 5, and with the formation of meristematic cell clumps and adventitious bud primordia, callus bands of 20 kDa and 55 kDa were clearly expressed , In the 20 kDa protein band contains pI of 5 5 ~ 65 specific protein. 43kDa with pI of 65 to 75 was the specific protein expressed in the early stage of adventitious buds. In this paper, the relationship between the organogenic ability of callus and the expressed protein was discussed.