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2012年对云南省腾冲市1例间日疟患者血样采用Care Start~(TM)疟疾快速诊断试剂盒、吉氏染色镜检和巢式PCR方法进行鉴定。Care Start~(TM)疟疾快速诊断试剂盒检测结果判定为除恶性疟原虫以外的间日疟原虫、三日疟原虫或卵形疟原虫感染。镜检结果显示,患者血样厚、薄血片中可见间日疟原虫环状体多核、多重感染现象,1个环状体有2个及以上核的占14.68%(188/1 280);同时寄生2个及以上环状体的红细胞占22.50%(288/1 280);1个红细胞内同时寄生环状体和滋养体、环状体和配子体。巢式PCR结果显示,患者血样为间日疟原虫特异性DNA片段阳性。结合检测结果、流行病学资料和临床表现,确诊该患者为输入性间日疟原虫感染病例,且一个红细胞内同时寄生不同期间日疟原虫。
In 2012, the blood samples of one Japanese malaria patient in Tengchong City of Yunnan Province were identified with Care Start ~ (TM) Rapid Diagnostic Kit, Giemsa staining and nested PCR. The test results of the Care Start ~ (TM) malaria rapid diagnostic kit were judged to be Plasmodium vivax, Plasmodium malaria or Plasmodium ovale other than Plasmodium falciparum. Microscopic examination showed that patients with blood thick, thin blood films can be seen in Plasmodium vivax multinuclear, multiple infections, a ring with two or more nuclei accounted for 14.68% (188/1 280); the same time The number of erythrocytes that parasitized two or more annuli accounted for 22.50% (288/1 280). One erythrocyte had simultaneous parasitic rings and trophozoites, rings and gametophytes. The results of nested PCR showed that the blood samples of patients were positive for Plasmodium vivax-specific DNA fragments. Combined with the test results, epidemiological data and clinical manifestations, the patient was confirmed as an imported case of Plasmodium vivax infection, and one of the erythrocytes parasitized Plasmodium vivax at different stages.