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目的 以小鼠胃癌细胞总RNA负载树突状细胞(DC),研究其抗肿瘤免疫作用。方法 磁性细胞分选器(MACS)自小鼠脾细胞中分选出CDllc+的DC作短期培养,以小鼠前胃癌细胞株的总RNA脉冲DC,分别以RNA脉冲的DC(RNA/DC)和未脉冲的DC(UDC)皮下注射免疫小鼠2次;免疫后7d,皮下接种小鼠前胃癌细胞MFC 5×105/鼠,以未作免疫注射的小鼠作为对照。接种肿瘤细胞后21d,处死实验动物,测量肿瘤体积,检测自然杀伤细胞(NK)、细胞毒性T淋巴细胞(CTL)活性和血清IL-12含量。结果 RNA/DC免疫组、UDC免疫组和对照组的皮下肿瘤体积分别为(0.3688±0.6571)、(0.7536±0.3659)、(2.6323±1.1435)cm3。RNA/DC免疫组外周血和脾细胞NK活性均明显升高,较对照组分别高出66.2％和65.4％;其肿瘤特异性CTL也明显升高,较对照组升高49.5％。UDC组NK和CTL活性也明显高于对照组。结论 短期培养的DC和以肿瘤细胞总RNA作为抗原脉冲的DC均可诱导较强的抗肿瘤免疫。
Objective To study the antitumor immunity of dendritic cells (DCs) loaded with total RNA of gastric cancer cells in mice. Methods DCs derived from CD11c + cells were isolated from mouse spleen cells by magnetic cell sorter (MACS). The DCs were pulsed with total RNA of mouse gastric cancer cell lines and stimulated with RNA pulsed DCs (RNA / DC) and Mice were immunized twice a day with unpulsed DC (UDC). Seven days after the immunization, mice were inoculated subcutaneously with MFC 5 × 105 / mouse, and mice without immunization were used as controls. Twenty-one days after the inoculation of tumor cells, the experimental animals were killed, the tumor volume was measured, the activity of natural killer cells (NK), cytotoxic T lymphocytes (CTL) and the content of IL-12 in serum were measured. Results The subcutaneous tumor volumes of RNA / DC immunized group, UDC immunized group and control group were (0.3688 ± 0.6571), (0.7536 ± 0.3659) and (2.6323 ± 1.1435) cm3, respectively. The NK activity of peripheral blood and spleen cells in RNA / DC group were significantly higher than those in control group (66.2% and 65.4%, respectively). The tumor-specific CTL was also significantly increased by 49.5% compared with control group. The activities of NK and CTL in UDC group were also significantly higher than those in control group. Conclusion Both DCs cultured short-term and DCs pulsed with total RNA of tumor cells can induce stronger anti-tumor immunity.