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目的:观察损伤血管内皮细胞生长因子(VEGF)的表达情况,探讨益气活血方对球囊损伤大鼠颈总动脉后再狭窄的干预机制。方法:随机将160只雄性SD大鼠分为假手术组,阴性对照组,益气活血方大、中、小剂量组。首先自左颈外动脉球囊损伤大鼠左颈总动脉,后给予高脂饲料喂养30 d,造成动脉硬化,然后经右股动脉穿刺球囊扩张损伤大鼠左颈总动脉,造成颈总动脉再狭窄。手术前1 d开始给药,每组动物于术后(3 d、7 d、14d、28 d)处死,取损伤颈总动脉,常规固定、包埋、切片,分别做HE染色和免疫组织化学SABC法检测VEGF的表达。结果:用药组VEGF表达明显增多,3 d、7 d和14 d益气活血方大剂量组VEGF表达量与阴性对照组、假手术组比较差异有显著性(P<0.05)。结论:益气活血方对球囊损伤后大鼠颈动脉再狭窄有明显的防治作用,其机制可能与上调VEGF的表达,促进血管内皮细胞修复与新生有关。
OBJECTIVE: To observe the expression of vascular endothelial growth factor (VEGF) and explore the intervention mechanism of Yiqi Huoxue decoction on carotid artery restenosis after balloon injury in rats. Methods: 160 male SD rats were randomly divided into sham operation group, negative control group, Yiqi Huoxue Fang large, medium and small dose group. First of all, the left carotid artery was injured from the left external carotid artery and then fed with high-fat diet for 30 days to cause atherosclerosis. Then, the right common carotid artery punctured the balloon and dilated the left common carotid artery to cause the common carotid artery Restenosis. The animals were sacrificed on the first day before operation (3 d, 7 d, 14 d and 28 d after operation). The common carotid arteries were fixed, embedded, and sectioned for HE staining and immunohistochemistry SABC method was used to detect the expression of VEGF. Results: The expression of VEGF in the treatment group was significantly increased. The expression of VEGF in the high dose Yiqi Huoxue prescription group was significantly lower than that in the negative control group and the sham operation group on the 3rd, 7th and 14th days (P <0.05). Conclusion: Yiqi Huoxue Prescription has obvious preventive and therapeutic effects on carotid restenosis after balloon injury in rats. The mechanism may be related to up-regulating the expression of VEGF and promoting the repair of vascular endothelial cells.