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用基因枪技术将雪花莲外源凝集素基因和大豆胰蛋白酶抑制剂基因,分别转化到水稻的胚性愈伤组织,经过筛选再生分别得到转基因Ⅰ和转基因Ⅱ植株.采用生物测定方法,并按照国际标准鉴定方法,将上述转基因稻株对褐稻虱、稻纵卷叶螟和三化螟进行了抗虫性鉴定,结果表明:(1)测试的转基因Ⅰ稻株中,对褐稻虱表现抗性的占测定总数的26.7%;(2)测试的转基因Ⅱ稻株中,对稻纵卷叶螟和三化螟表现抗性的,分别占测定总数的30.18%和1.5%;(3)上述转基因稻株与原种(台北309)及感虫对照种(TN1或Rexoro)对比,后者皆表现高感或感虫,而前者均表现出具有不同程度的抗虫性,还出现了不少高抗的株系,说明转基因稻株已从外源基因中获得了抗虫性,该研究已成功地进行了抗虫稻株的基因转化
The gene of snowdrop lectin gene and soybean trypsin inhibitor gene were respectively transformed into embryogenic callus of rice by gene gun technology, and the transgenic Ⅰ and the transgenic Ⅱ plants were obtained through screening and regeneration respectively. The bioassay method was used to identify the resistance of B. tabaci to the rice brown planthopper and rice stem borer, Chilo suppressalis (Walker). The results showed that: (1) The transgenic rice plants tested , The resistance to brown planthopper resistance accounted for 26.7% of the total number of measurements; (2) The tested transgenic rice plants resistant to the rice leaf roller and the rice stem borer, respectively, accounted for the total number of measurements 30.18% and 1.5% respectively. (3) Compared with the original species (Taipei 309) and the susceptible species (TN1 or Rexoro), the above transgenic rice plants showed high susceptibility or susceptible to insects, There are also many highly resistant strains that have emerged, indicating that transgenic rice plants have obtained insect resistance from exogenous genes. This study has successfully carried out the transformation of insect-resistant rice strains