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目的：观察大鼠肝脏缺血再灌注损伤后内源性转化生长因子β（ＴＧＦβ）和碱性成纤维细胞生长因子（ｂＦＧＦ）基因表达的变化规律，探讨这两种生长因子在缺血再灌注所致内脏损伤中作用，为临床防治缺血再灌注损伤提供理论基础。方法：实验采用原位杂交和逆转录聚合酶链式反应技术，观察两种生长因子的ｍＲＮＡ表达水平。结果：实验发现，正常大鼠肝细胞和肝血窦中存在少量ＴＧＦβｍＲＮＡ，而ｂＦＧＦｍＲＮＡ含量较高；在缺血４５分钟时，ＴＧＦβ基因表达略有增加，而ｂＦＧＦ略减少；再灌注６小时后，ＴＧＦβｍＲＮＡ表达显著增高，ｂＦＧＦ较正常对照大鼠也增加；再灌注２４小时后，ＴＧＦβ和ｂＦＧＦ均恢复至正常。结论：大鼠肝脏缺血再灌注损伤后，内源性ＴＧＦβ和ｂＦＧＦ的ｍＲＮＡ表达量随损伤时间不同而变化，这两种生长因子可能参与缺血再灌注所致的内脏损伤后的修复作用
OBJECTIVE: To observe the changes of gene expression of transforming growth factor beta (TGFβ) and basic fibroblast growth factor (bFGF) after hepatic ischemia-reperfusion injury in rats and to explore the role of these two growth factors in ischemia-reperfusion Resulting in the role of visceral injury, provide a theoretical basis for the prevention and treatment of ischemia-reperfusion injury. Methods: In situ hybridization and reverse transcription polymerase chain reaction (PCR) were used to observe the mRNA expression of two growth factors. Results: The results showed that a small amount of TGFβ mRNA was present in normal rat hepatocytes and hepatic sinusoids, whereas the content of bFGF mRNA was higher. At 45 minutes of ischemia, TGFβ mRNA expression increased slightly and bFGF slightly decreased. After 6 hours of reperfusion, TGFβmRNA expression was significantly increased, bFGF than normal control rats also increased; 24 hours after reperfusion, TGFβ and bFGF returned to normal. CONCLUSION: The mRNA expression of endogenous TGFβ and bFGF after liver ischemia reperfusion injury in rats varies with the time of injury, and these two growth factors may participate in the repair after visceral injury induced by ischemia-reperfusion