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目的:研究地塞米松(dexamethasone)对小鼠骨髓源树突状细胞(bone marrow-derived dendritic cells,BMDC)表型及功能的影响。方法:用GM-CSF及IL-4定向诱导C57BL/6小鼠骨髓细胞分化DC,分化过程中随机分为4组,A组,对照组;B、C、D组分别为100μg/L地塞米松组,100μg/LLPS组,100μg/L地塞米松+100μg/LLPS组。每组细胞培养8d。用流式细胞术测定BMDC表面CD80,CD86,Galectin-9及PD-L1的表达,ELISA方法检测其培养上清IL-12的浓度,用混合淋巴细胞培养法检测其对同种异体T细胞的刺激能力。结果:与对照组比较,LPS刺激后,DC表面CD80,CD86,Galectin-9及PD-L1表达明显增加,培养上清中IL-12浓度升高,其刺激同种异体T细胞的能力增强。与对照组和LPS组比较,地塞米松可使DC表面CD80,CD86,Galectin-9及PD-L1表达明显降低,IL-12分泌减少,刺激同种异体T细胞的能力减弱。结论:地塞米松降低了BMDC细胞表型表达,抑制其细胞因子IL-12的分泌,降低其刺激同种异体反应T细胞增殖的能力。
Objective: To study the effect of dexamethasone on the phenotype and function of mouse bone marrow-derived dendritic cells (BMDC). Methods: The bone marrow cells of C57BL / 6 mice were induced to differentiate into DCs by targeting GM-CSF and IL-4. The cells were randomly divided into 4 groups, A group and control group. B, C and D groups were treated with 100μg / 100 mg / L dexamethasone + 100 μg / L LPS group. Each group of cells cultured 8d. The expression of CD80, CD86, Galectin-9 and PD-L1 on BMDC surface were measured by flow cytometry. The concentration of IL-12 in supernatant of culture supernatant was measured by ELISA. The effect of allogeneic T cells Stimulating ability. Results: Compared with the control group, the expression of CD80, CD86, Galectin-9 and PD-L1 on the surface of DCs were significantly increased after LPS stimulation. The concentration of IL-12 in the culture supernatant increased and the ability of stimulating allogeneic T cells was enhanced. Compared with the control group and the LPS group, dexamethasone significantly reduced the expression of CD80, CD86, Galectin-9 and PD-L1 on the DC surface, decreased the secretion of IL-12, and weakened the ability of stimulating allogeneic T cells. Conclusion: Dexamethasone can reduce the phenotype of BMDC cells, inhibit the secretion of IL-12 and decrease the ability of stimulating the proliferation of allogenic T cells.