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[目的 ]调查自然环境中致泻性大肠埃希氏菌的分布、致病性与药物敏感性 ,为制定预防策略选择有效治疗药物提供科学依据。 [方法 ] 1999年 1月至 2 0 0 1年 4月检测石家庄市不同环境样品 ,对分离的致泻性大肠埃希氏菌株进行动物实验和药物敏感性测定。 [结果 ]检测各类样本 886份 ,检出 10 3株致泻性大肠埃希氏菌 (检出率为 11 63 % ) ,其中EPEC 71株 11个血清型 ,EIEC 2 6株 6个血清型 ,ETEC 5株 3个血清型 ,EHEC 1株为O157∶H7血清型 ;从鸡肠腔内容物、带鱼、草鱼体中分离出的O2 5∶K19血清型ETECLT阳性 ,从蟹、鱿鱼体中检出的O8∶K40 、O78∶K80 血清型ETEC既产生LT又产生ST。 2 6株EIEC均有侵袭力 ,O157∶H7致小白鼠发病死亡。全部菌株对新霉素、亚胺硫霉素、头孢哌酮敏感。 [结论 ]石家庄市各种环境样品中均可检出致泻性大肠埃希氏菌 ,应采取措施 ,防止污染环境与食品 ,预防食源性疾病
[Objective] To investigate the distribution, pathogenicity and drug sensitivity of diarrhea-causing Escherichia coli in the natural environment and provide a scientific basis for the development of preventive strategies to select effective therapies. [Method] From January 1999 to April 2001, different environmental samples were tested in Shijiazhuang City. Animal experiments and drug sensitivity tests of isolated diarrheal Escherichia coli strains were carried out. [Results] A total of 886 specimens of various types were detected, of which 103 strains were confirmed as Escherichia coli (detection rate: 11 63%). There were 11 strains of EPEC in 71 strains and 6 strains of EIEC in 26 strains , ETEC 5 strains of 3 serotypes, EHEC 1 strain of O157: H7 serotypes; from the intestine cavity contents, octopus, grass carp body isolated O2 5:K19 serotype ETECLT positive, from crab, squid body seized Out of O8: K40, O78: K80 serotype ETEC both produce LT and produce ST. 26 strains of EIEC are invasive, O157: H7 induced death in mice. All strains of neomycin, imipenem, cefoperazone sensitive. [Conclusion] The samples of diarrhea-causing Escherichia coli could be detected in various environmental samples of Shijiazhuang City. Measures should be taken to prevent the pollution of the environment and food, prevention of food-borne diseases