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优化超声波提取玉竹多糖及脱色处理的工艺。在单因素试验的基础上,通过响应面法、正交试验法分别对玉竹多糖的超声波提取及脱色工艺进行优化。超声波提取的最佳工艺条件为超声功率359 W,超声时间21 min,料液比1︰20(g/m L),试验结果所得玉竹多糖得率为5.17%,与预测值吻合;活性炭脱色最佳工艺为活性炭用量1.5 g,p H为7,在70℃下震荡30 min,脱色率为28.44%,多糖剩余率为97.21%。优选得到的超声波提取工艺简单易行,稳定性好。脱色后多糖剩余率高,可作为多糖初步纯化的方法。
Optimization ultrasonic extraction Polygonatum polysaccharides and bleaching process. On the basis of single factor experiment, the ultrasonic extraction and decoloration technology of Polygonatum polysaccharide were optimized by response surface method and orthogonal test method respectively. The optimum conditions of ultrasonic extraction were ultrasonic power of 359 W, ultrasonic time of 21 min and solid-liquid ratio of 1: 20 (g / m L). The yield of Polygonatum polysaccharide was 5.17%, which was consistent with the predicted value. The optimum conditions were activated carbon 1.5 g, pH 7, shaking at 70 ℃ for 30 min, the decolorization rate was 28.44% and the polysaccharide remaining rate was 97.21%. The preferred ultrasonic extraction process is easy and stable. After the bleaching polysaccharide residual rate is high, can be used as a method of preliminary purification of polysaccharides.