论文部分内容阅读
目的:构建高通量组织微阵列(tissuemicroarray)/组织芯片(tissuechip),检测其上IGFⅡ的表达情况,以明确IGFⅡ表达与肺癌的相关性。方法:用组织阵列仪制备按不同组织学类型排布的组织芯片,然后用免疫组织化学SP法检测一张组织芯片上54例肺癌肿瘤组织和10例正常肺组织样本中的IGFⅡ表达情况,并分析其表达与各临床病理学参数之间的相关性。结果:IGFⅡ蛋白在实验组中的阳性表达率为42.6%(23/54),对照组为阴性,两者之间具有显著性差异(P<0.05);IGFⅡ表达阳性率与大体类型、组织学分级、临床分期有关(P<0.05),而与性别、年龄、组织学分型及淋巴结转移无关(P>0.05)。结论:IGFⅡ的表达与肺癌的恶性行为有关,检测此指标对预测肺癌的预后和指导治疗有一定的参考价值。组织芯片是一较高的技术平台,可以应用此技术进行大规模组织样本的检测。
OBJECTIVE: To establish a high-throughput tissue microarray / tissue chip to detect the expression of IGFⅡ and to clarify the correlation between IGFⅡ expression and lung cancer. Methods: Tissue microarrays were prepared by tissue array according to different histological types. The expression of IGF Ⅱ in 54 lung cancer tissues and 10 normal lung tissues from a tissue microarray was detected by immunohistochemical SP method. The correlation between the expression and the clinicopathological parameters was analyzed. Results: The positive rate of IGFⅡ protein in the experimental group was 42.6% (23/54), while it was negative in the control group (P <0.05). The positive rate of IGFⅡ was positively correlated with the gross type and histological score Grade and clinical stage (P <0.05), but not with sex, age, histological type and lymph node metastasis (P> 0.05). Conclusion: The expression of IGF Ⅱ is related to the malignant behavior of lung cancer. Detecting this index has some reference value for predicting the prognosis and guiding treatment of lung cancer. Tissue chips are a high technology platform that can be used for large-scale tissue sample detection.