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目的为制备分泌抗日本血吸虫感染保护性单克隆抗体细胞株。方法应用杂交瘤技术，将照射致弱尾蚴免疫ＢＡＬＢ／ｃ小鼠的脾细胞与小鼠骨髓瘤的细胞融合。结果经两次以上有限稀释克隆化后，抗日本血吸虫童虫表膜抗原阳性率为１００％，建立了５株稳定分泌抗童虫表膜单抗的细胞株。杂交瘤细胞诱生腹水，ＥＬＩＳＡ效价达１０．６４×１０５～１１．２８×１０５。初步鉴定单克隆抗体类型分别为ＩｇＭ、ＩｇＧ２ａ和ＩｇＧ２ｂ。抗原抗体定位于童虫表膜。结论该单抗的制备为进一步研制抗日本血吸虫童虫表膜分子疫苗奠定基础。
The purpose of preparation for the secretion of anti-Japanese schistosome infection protective monoclonal antibody cell line. Methods The hybridoma technique was used to fuse the splenocytes of BALB / c mice immunized with irradiated cercariae with mouse myeloma cells. Results After being cloned twice or more in a limited dilution, the positive rate of the surface antigens of Schistosoma japonicum was 100%, and five cell lines stably secreting the anti-spp surface McAb were established. Ascites was induced by hybridoma cells with ELISA titer of 10.64 × 105 ~ 11.28 × 105. Preliminary identification of monoclonal antibody types were IgM, IgG2a and IgG2b. Antigen antibodies located in the Schistosoma surface membrane. Conclusion The preparation of this monoclonal antibody lays the foundation for further development of molecular vaccine against Schistosoma japonicum schistosomula.