论文部分内容阅读
目的研究不同亚型乙型肝炎患者外周血T淋巴细胞亚群变化及其临床意义。方法选择40例慢性活动性乙型肝炎、30例乙型肝炎病毒携带者、30例乙型肝炎肝硬化、20例急性乙型肝炎患者和30例健康对照者,采用流式细胞仪检测各组外周血T淋巴细胞亚群的表型和频率。结果与正常对照组比较,慢性活动性肝炎、肝炎肝硬变患者外周血CD4+、CD4+/CD8+比值均有显著性下降(P<0.01),以肝炎肝硬变下降最显著,慢性病毒携带者肝炎、急性乙型肝炎外周血T细胞亚群数量和比值变化不明显(P>0.05)。HBV DNA病毒载量与患者外周血CD4 T细胞数量无相关性(P>0.05)。结论慢性活动性乙型肝炎和肝硬化患者的细胞免疫功能降低,其下降程度与病毒复制水平无相关性。
Objective To study the changes of T lymphocyte subsets in peripheral blood of patients with different subtypes of hepatitis B and its clinical significance. Methods 40 cases of chronic active hepatitis B, 30 cases of hepatitis B virus carriers, 30 cases of hepatitis B cirrhosis, 20 cases of acute hepatitis B patients and 30 healthy controls were selected, and each group was detected by flow cytometry Phenotype and frequency of peripheral blood T lymphocyte subsets. Results Compared with the control group, the ratio of CD4 + and CD4 + / CD8 + in peripheral blood of patients with chronic active hepatitis and cirrhosis of liver were significantly decreased (P <0.01), and the most significant decrease was liver cirrhosis of hepatitis. Chronic viral hepatitis There was no significant change in the number and ratio of T lymphocyte subsets in peripheral blood of patients with acute hepatitis B (P> 0.05). There was no correlation between HBV DNA viral load and the number of CD4 T cells in peripheral blood (P> 0.05). Conclusion The cellular immune function of patients with chronic active hepatitis B and cirrhosis is decreased, and the degree of decrease is not correlated with the level of virus replication.