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NAC(NAM-ATAF-CUC)转录因子在植物逆境胁迫应答反应中发挥重要作用。通过同源克隆方法从长穗偃麦草中分离到一个NAC转录因子基因EeNAC9(Elytrigia elongate NAM-ATAF-CUC 9),该基因编码274个氨基酸,等电点为9.03;对该蛋白二级结构预测发现主要存在9个螺旋区(helices)和无规则卷曲(coils)。氨基酸疏水性分析表明,该蛋白存在大量的疏水性氨基酸。将该基因构建到PBI121双元植物表达载体上,通过农杆菌介导转化烟草,经PCR分子检测获得T0代35S::EeNAC9转基因烟草阳性植株65株。胁迫培养基上初步功能研究表明,过表达EeNAC9转基因烟草表现出对ABA的敏感性,同时增强了对干旱、高盐的胁迫耐性,从而为小麦抗逆分子育种提供了优良候选基因资源。
NAC (NAM-ATAF-CUC) transcription factors play an important role in plant stress response. A NAC transcription factor gene EeNAC9 (Elytrigia elongate NAM-ATAF-CUC 9) was isolated from Erechophila elongatus by homologous cloning method. The gene encoded 274 amino acids with an isoelectric point of 9.03. The secondary structure of the protein was predicted It is found that there are mainly 9 helices and irregular coils. Amino acid hydrophobicity analysis shows that the protein has a large number of hydrophobic amino acids. The gene was constructed into the PBI121 binary plant expression vector and transformed into tobacco by Agrobacterium tumefaciens. T0 generation 35S :: EeNAC9 transgenic tobacco plants were detected by PCR. Preliminary functional studies on stress media showed that EeNAC9 overexpression transgenic tobacco showed sensitivity to ABA and enhanced its resistance to drought and high salinity, thus providing excellent candidate gene resources for wheat resistance to molecular breeding.