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利用随机扩增多态性技术(RAPD)研究生姜连作地、施加有机肥后的生姜连作地及新开地中土壤微生物多样性与生姜青枯病的关系,结果发现,连作地的青枯病发病率为100%,新开地没有发病,而施加有机肥的连作地生姜青枯病发病率降至37%。发现施加有机肥连作地的真菌、放线菌数量增加,平板上不同菌落形态的真菌、细菌、放线菌增加明显。多样性指数分析发现Shannon指数、Simpson指数等均显著增加。随机扩增条带出现连作地的条带丰富度较少、新开地的条带最多、施加有机肥连作地的RAPD条带明显多于连作地,接近新开地,并出现了一些连作地没有的特异条带。结果表明,施加有机肥可以改善土壤微生物群落结构。
Using random amplified polymorphic (RAPD) technique to study the relationship between soil microbial diversity and ginger bacterial wilt after continuous cropping with ginger and organic manure after application of organic fertilizer, it was found that even the bacterial wilt The incidence rate was 100%. There was no new disease in the newly opened land. However, the incidence of continuous bacterial wilt of organic fertilizer was reduced to 37%. It was found that the amount of actinomycetes increased with the continuous application of organic fertilizers, and the fungi, bacteria and actinomycetes with different colony morphology on the plate increased significantly. The diversity index analysis found that Shannon index and Simpson index increased significantly. The band of random amplified bands appeared to be less abundant, with the largest number of newly opened bands. The RAPD bands of continuous cropping with organic fertilizers were significantly more than those of continuous cropping and close to newly opened ones, No special strip. The results showed that applying organic manure could improve the microbial community structure.