目的:为了明确贮脂细胞(Fat Storing Cell,FSC)和肝细胞(Hepatocyte,HC)在肝纤维化胶原合成中的作用.方法:本文在分离培养大鼠FSC和HC的基础上,用~3H-Proline掺入结合胶原酶消化法和定量斑点杂交法,分析了体外培养的FSC和HC胶原和非胶原蛋白的合成以及Ⅰ型前胶原mRNA的稳定性.结果:发现FSC合成胶原蛋白的能力较HC强,而非胶原蛋白则与此相反;FSC中Ⅰ型前胶原mRNA较HC中稳定,且半衰期较长.结论:FSC在ECM胶原合成中可能起主要作用,但HC的作用也不容忽视. Objective: To clarify the role of fatty storing cell (FSC) and hepatocyte (Hepatocyte, HC) in collagen synthesis of hepatic fibrosis.Methods: Based on the isolation and culture of rat FSC and HC, -Proline incorporation and collagenase digestion and quantitative dot blot hybridization were used to analyze the synthesis of collagen and non-collagen FSC and HC in vitro and the stability of type Ⅰ procollagen mRNA.Results: The ability of FSC to synthesize collagen was better than that of collagen HC was stronger than non-collagen, whereas type I procollagen mRNA in FSC was more stable than HC and had a longer half-life.Conclusion: FSC may play a major role in ECM collagen synthesis, but the role of HC should not be overlooked.