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目的 :采用信息探针获取正常肝、肝旁组织和肝癌HCC XmRNA的表达信息 ;采用NMR1H谱分析技术捕获非癌性肝占位相关Pr谱学特征 ,探讨其规律和临床价值 .方法 :将获得的全长基因用u 12 P DATP标记为探针 ,用3 2 P HCC X杂交MTNblot膜片进行Westernblot检测基因表达功能 ;用NMR1H谱仪分析 19种单体氨基酸的化学位移等参数 ,以《Handbookofproton NMRspectraandData》氨基酸图谱为参比 ,设置适宜的解析条件 .结果 :转染BEL74 0 2肝癌细胞的体外克隆形成实验表明 ,HCC X对其细胞在体内生长有明显抑制作用 ,在肝癌组织中为低表达或无表达 ,与正常组织比较有显著性差异P <0 .0 1;NMR1H分析 19种单体aa,14种完全相符 ,1种相似 ,TrP和HiS分别为首次获得 .结论 :HCC X可能是肝癌的致病基因 ,与其高度同源性的HFARP具有抑制血管内皮细胞凋亡的作用 ;NMR1H谱分析相关标志Pr的条件可满足指纹区信息捕获要求 ,为深入探讨病生机制相关研究提供新的方法学
OBJECTIVE: To obtain the expression information of HCC XmRNA in normal liver tissues, liver tissues and hepatocellular carcinoma using information probes and to capture the characteristics of non-cancerous liver space-related Pr spectra by using NMR1H spectral analysis.Methods: Of the full-length gene was labeled with u 12 P DATP as a probe, with 3 2 P HCC X hybridization MTNblot membrane Western blot detection of gene expression function; NMR1H spectrometer chemical analysis of 19 kinds of monomer amino acid shift parameters, "Handbookofproton NMR spectra and NMR spectra were used as reference.Results: The results of in vitro cloning experiments of transfected BEL74 0 2 hepatocarcinoma cells showed that HCC X significantly inhibited the growth of its cells in vivo and low expression in HCC tissues Or no expression, compared with normal tissue P <0.01; NMR1H analysis of 19 kinds of monomers aa, 14 kinds of exactly the same, a similar, TrP and HiS were first obtained.Conclusion: HCC X may be The pathogenic genes of hepatocellular carcinoma and its highly homologous HFARP have the effect of inhibiting the apoptosis of vascular endothelial cells; NMR1H spectral analysis of the relevant conditions of Pr can meet the fingerprint information capture requirements , To provide new methodologies for further research on the pathogenesis of disease