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目的：探讨鼻咽组织、外周血白细胞ＥＢＶ基因组分布及与ＮＰＣ的关系。方法：采用ＰＣＲ法检测２６例ＮＰＣ，４例癌旁及１４例慢性鼻咽炎鼻咽组织及外周血白细胞中ＥＢＶＤＮＡ。同时采用酶免疫法测定血清ＶＣＡ－ＩｇＡ。结果：１．ＮＰＣ、癌旁及鼻咽炎组织ＥＢＶＤＮＡ无明显差异。２．ＮＰＣ与鼻咽炎组外周血白细胞ＥＢＶＤＮＡ有明显差异，Ｐ＜０．０５。３．ＮＰＣ与鼻咽炎组血清ＶＣＡ－ＩｇＡ之间存在明显差异。Ｐ＜０．０５。４．同时检测以上三项后三项阳性率及二项阳性率在两组间有显著差异。Ｐ＜０．０５。５．ＮＰＣ活检组织及血ＥＢＶＤＮＡ与血清ＶＣＡ－ＩｇＡ阳性率之间有良好的一致性。结论：ＮＰＣ鼻咽组织存在大量的ＥＢＶＤＮＡ拷贝数，少数分布于外周血白细胞，且与血清ＶＣＡ－ＩｇＡ有良好的一致性。３项套式检测比单项鼻咽活检组织ＥＢＶＤＮＡ或血清ＶＣＡ－ＩｇＡ更有助于对ＮＰＣ的诊断。
Objective: To investigate the distribution of EBV genome in nasopharyngeal and peripheral leukocytes and its relationship with NPC. Methods: EBV DNA was detected by PCR in 26 cases of NPC, 4 cases of paracancerous tissues and 14 cases of chronic nasopharyngitis nasopharyngeal tissues and peripheral white blood cells. At the same time, serum VCA-IgA was measured by enzyme immunoassay. Results: 1. NPC, adjacent and nasopharyngitis EBV DNA no significant difference. 2. EBV DNA of peripheral blood leukocytes in NPC and nasopharyngitis group were significantly different, P <0.05.3. NPC and nasopharyngitis serum VCA-IgA significant difference between. P <0.05.4. At the same time detection of the above three positive rate and positive rate of two significant differences between the two groups. P <0.05.5. There was a good agreement between NPC biopsies and blood EBVNA and serum VCA-IgA positive rates. Conclusion: There are a large number of copies of EBV DNA in nasopharyngeal tissues of NPC, a few are distributed in peripheral leukocytes and have good consistency with serum VCA-IgA. 3 sets of nested detection than nasopharyngeal biopsy EBVDNA or serum VCA-IgA is more helpful for the diagnosis of NPC.