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作者通过体外培养证实了粒系造血和巨噬细胞的产生是由正反馈机理包括灶刺激因子(CSF)所调控,而这种正反馈又被负反馈(抑素)作用平衡,甚至提出在粒系造血暂时调节过程中负反馈更为重要。本文主要探讨了在造血的调节机理中,前列腺素(PGE)的作用。实验用年龄2～3月的C57BI/6小鼠,用稍加改进的体外琼脂培养技术(1份3%琼脂,9份含15%小牛血清改进的McCoy′S 5A培养基。加入鼠粒-单核细胞型白血病细胞系(WEHI-3)培养的CSF),观察骨髓定向粒-巨噬祖细胞(CFU-C)、脾B-淋巴
The authors demonstrated in vitro that the generation of myeloid hematopoiesis and macrophages is regulated by a positive feedback mechanism, including the focal stimulus (CSF), which is in turn balanced by negative feedback (somatostatin) Department of hematopoietic regulation of negative feedback is more important. This article explores the role of prostaglandin (PGE) in the regulation of hematopoiesis. Experimental C57BI / 6 mice, aged 2-3 months, were treated with a slightly modified in vitro agar culture technique (1 part 3% agar, 9 parts McCoy’S 5A medium modified with 15% calf serum. - monocyte-type leukemia cell line (WEHI-3)), bone marrow-derived macrophage progenitor cells (CFU-C), spleen B-lymphoid