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以甜樱桃优良极晚熟新品种“晚红珠”的嫩梢为外植体,通过使用大蒜溶液对外植体的灭菌以及初代培养、继代培养、生根培养和组培苗驯化移栽等条件的研究,确定了甜樱桃优良极晚熟新品种“晚红珠”的离体再生技术体系。表明:1:1(W/V)大蒜水溶液灭菌30 min,灭菌效果最好,所接种的嫩梢平均无菌率为100%;初代培养适宜的培养基为:MS+6-BA 0.2 mg/L+IBA 0.05 mg/L+琼脂7 g/L+蔗糖30 g/L,初代培养的萌芽率可达93%;以MS+6-BA 0.5 mg/L+IAA 0.1 mg/L,附加琼脂7 g/L,蔗糖30 g/L,茎段增殖系数为最大,平均为5.6;以MS+6-BA 0.1 mg/L+IBA 0.5 mg/L,附加琼脂7 g/L,蔗糖30 g/L,生根最好,生根率达到100%,生根条数平均为12.5条;在组培苗的驯化过程中,中性壤土中的成活率为90.50%,而珍珠岩和沙土上的效果更好,存活率均可达到100%。
Using the young shoots of “sweet red pearl”, which is a very late-maturing sweet cherry variety, as explants, the explants were sterilized and primary cultured using garlic solution, followed by subculture, rooting and tissue culture seedling transplanting And other conditions, to determine sweet cherry excellent late late maturity “late red beads ” in vitro regeneration technology system. The results showed that the sterilizing effect of 1: 1 (W / V) garlic aqueous solution for 30 min was the best, and the average sterile rate of inoculated shoots was 100%. The suitable culture medium of primary culture was MS + 6-BA 0.2 The germination rate of primary culture was up to 93% with 0.05 mg / L IBA 0.05 mg / L + agar 7 g / L + sucrose 30 g / L, and the medium with MS + 6- BA 0.5 mg / L + IAA 0.1 mg / g / L, sucrose 30 g / L, and the average multiplication coefficient of stem was 5.6. MS + 6-BA 0.1 mg / L + IBA 0.5 mg / L was added with agar 7 g / L and sucrose 30 g / , Rooting best, the rooting rate reached 100%, the average rooting number of 12.5; in the process of tissue culture seedlings in the neutral loam survival rate of 90.50%, while perlite and sand on the better, Survival rate can reach 100%.