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1985年8～9月广州市发生以发热、头痛、关节痛、乏力、皮疹等为临床表现的疾病流行。我们用C_6/36白纹伊蚊细胞株从6例发病1～3天的急性期病人血清中分离得5株病毒。从流行区捕获的三组白纹伊蚊分离到1株病毒。用登革热Ⅰ～Ⅳ型单克隆抗体间接免疫荧光法,补体结合试验,以及患者双份血清抗体测定,均鉴定为Ⅰ型登革热病毒。这是广州市首次从白纹伊蚊分离得Ⅰ型登革热病毒。试验结果还表明,Ⅰ～Ⅳ型登革热单克隆抗体间接免疫荧光法对登革热病毒分型鉴定,具有特异、灵敏、快速的优点。
From August to September 1985, a epidemic of clinical manifestations of fever, headache, joint pain, fatigue and rash occurred in Guangzhou City. We used C_6 / 36 Aedes albopictus cell line from 6 cases of onset of 1 to 3 days in the acute phase of the patient’s serum isolated five viruses. One strain of virus was isolated from three groups of Aedes albopictus captured from endemic areas. Indirect immunofluorescence, complement fixation test, and double serum antibody determination of dengue Ⅰ ~ Ⅳ monoclonal antibodies were all identified as type Ⅰ dengue virus. This is the first time that Guangzhou has isolated dengue virus type Ⅰ from Aedes albopictus. The test results also showed that the indirect immunofluorescence method of dengue monoclonal antibody (Ⅰ ~ Ⅳ) has the advantage of specificity, sensitivity and rapidness to identify the dengue virus.