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以野生青蒿中形态差异较为明显的绿秆(品系Y03)和紫红秆(品系Y07)为材料,分别以碘-碘化钾染色法、TTC染色法、联苯胺-甲萘酚染色法和离体萌发法测定其花粉活力,并从中选取较适宜的检测方法研究其贮藏特性,采用联苯胺-过氧化氢法测定其柱头的可授性.结果表明,碘-碘化钾和TTC染色法不适宜青蒿的花粉活力检测,联苯胺-甲萘酚染色法可以用于快速测定青蒿花粉活力,但测定值偏高,离体萌发法能准确可靠地反应花粉活力;两个品系的新鲜花粉在室温条件下只能贮藏2d,4℃,-19℃条件下可以贮藏3d,干燥处理显著加快了花粉活力的丧失;联苯胺-过氧化氢法测得两个品系青蒿柱头可授期为8~10d,边缘雌花可授性比中央两性花的强,且其可授期比中央两性花长1~2d.
The green stalk (line Y03) and purple stalk (line Y07) with obvious morphological differences in wild A. annua were used as materials, and were stained with iodine-potassium iodide, TTC, benzidine-a-naphthol and in vitro germination The pollen viability was determined and the suitable storage method was selected to study its storage characteristics.The stigma receptivity was determined by benzidine-hydrogen peroxide method.The results showed that iodine-potassium iodide and TTC staining were not suitable for Artemisia annua L. Pollen viability test and benzidine-a-naphthol staining could be used to determine the pollen viability quickly, but the measured value was too high. The in vitro germination method could accurately and reliably reflect the pollen viability. Fresh pollen of two cultivars at room temperature Can only be stored for 2d, can be stored for 3d at -19 ℃ under the condition of -19 ℃, the loss of pollen viability can be significantly accelerated by drying treatment. The stigma of Artemisia annua L. obtained by the method of benzidine-hydrogen peroxide can be 8 ~ 10d, Peripheral female flowers can be granted more than the central bisexual flowers, and its duration can be granted than the central flowers spent 1 ~ 2d.