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目的:探讨四逆汤在阿霉素(ADR)诱导的心力衰竭中的心肌保护作用及其可能的机制。方法:SD大鼠随机分为正常对照组、模型组及四逆汤组,模型组与四逆汤组大鼠尾静脉注射ADR复制心衰模型,四逆汤组给予四逆汤煎剂灌胃,其生药含量为3.75 g/(kg·d),实验结束时留取各组大鼠心肌组织,以免疫组化法测定心肌组织Bcl-xl蛋白及Bid蛋白表达情况,RT-PCR法检测Bcl-xl/Bcl-xs基因表达情况,流式细胞术测定心肌细胞凋亡情况。结果:与正常对照组比较,模型组大鼠心肌组织Bcl-xl蛋白的阳性反应物显著减少,Bid蛋白的阳性反应物显著增多,RT-PCR结果表明模型组Bcl-xl与Bcl-xs基因表达的比值与正常对照组比较显著下降,心肌细胞凋亡增多:而应用四逆汤后Bcl-xl蛋白阳性反应物表达显著上调,Bid蛋白表达下降,凋亡减少,Bcl-xl和Bcl-xs基因表达的比值则接近正常对照组。结论:Bcl-xl在阿霉素所导致的心衰发病中可能占有重要作用,四逆汤可能是通过上调抑凋亡因子Bcl-xl的表达,下调促凋亡因子Bcl-xs和Bid的表达来保护心肌细胞的。
Objective: To investigate the myocardial protective effect of Sini decoction in heart failure induced by adriamycin (ADR) and its possible mechanism. Methods: Sprague-Dawley rats were randomly divided into normal control group, model group, and Sini Decoction group. Rats in the model group and Sini Decoction group were injected with ADR to replicate the heart failure model. Sini Decoction group was given Sini Decoction by intragastric administration. The crude drug content was 3.75 g/(kg·d). At the end of the experiment, the myocardial tissue was obtained from each group. The expression of Bcl-xl protein and Bid protein in myocardial tissue was detected by immunohistochemistry. Bcl was detected by RT-PCR. The expression of -xl/Bcl-xs gene was detected by flow cytometry. RESULTS: Compared with the normal control group, the positive reaction products of Bcl-xl protein in the myocardial tissue of the model group were significantly reduced, and the positive reaction products of the Bid protein were significantly increased. RT-PCR results showed that the Bcl-xl and Bcl-xs gene expression in the model group. Compared with the normal control group, the ratio of the ratio was significantly decreased and cardiomyocyte apoptosis was increased: while the expression of Bcl-xl protein positive reaction was significantly up-regulated, the expression of Bid protein was decreased, apoptosis was decreased, and Bcl-xl and Bcl-xs genes were applied after Sini Decoction. The ratio of expression is close to the normal control group. Conclusion: Bcl-xl may play an important role in the pathogenesis of heart failure caused by doxorubicin. Sini Decoction may down-regulate the expression of Bcl-xl and down-regulate the expression of Bcl-xs and Bid. To protect the heart muscle cells.