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斑马纹病是剑麻生产上最严重、毁灭性病害之一。本研究基于寄生疫霉菌多聚半乳糖醛酸酶pppg6~pppg10等5个基因c DNA序列设计了各基因编码区的特异引物。利用5对引物分别对不同来源的剑麻斑马纹病菌DNA进行了分子检测。检测结果表明,在被检测的剑麻斑马纹病菌菌株中均存在与寄生疫霉菌多聚半乳糖醛酸酶pppg6~pppg10对应的基因Szpg6~Szpg10。基因序列比对分析表明,来自斑马纹病菌的Szpg6、Szpg8基因与对应pppg6、pppg8基因之间序列高度一致。比较而言,来自斑马纹病菌的Szpg7、Szpg9以及Szpg10基因与对应同源基因之间分别存在3、6及4个核苷酸序列差异,进而导致3、5及3个推定氨基酸的变异。由此推测,来自斑马纹病菌的Szpg7、Szpg9及Szpg10基因可能与对应基因在功能上存在着一定的差异。本研究为进一步研究剑麻斑马纹病菌多聚半乳糖醛酸酶在致病过程中的作用奠定了基础。
Zebra pattern is one of the most serious and devastating diseases in sisal production. In this study, specific primers of each gene coding region were designed based on the five gene c DNA sequences of Phytophthora infestans polygalacturonase pppg6 ~ pppg10. Five pairs of primers were used to detect the DNA of S. sinensis from different sources. The results showed that Szpg6 ~ Szpg10, which corresponds to Phytophthora infestans polygalacturonase pppg6 ~ pppg10, existed in the tested strains of S. sinensis. Sequence alignment analysis showed that the sequences of Szpg6 and Szpg8 from zebra were highly consistent with those of pppg6 and pppg8. In comparison, there are 3, 6 and 4 nucleotide sequence differences between Szpg7, Szpg9 and Szpg10 genes and corresponding homologous genes respectively from Zebra pattern bacteria, resulting in 3, 5 and 3 putative amino acid variations. Therefore, it is speculated that the Szpg7, Szpg9 and Szpg10 genes from zebra may be functionally different from the corresponding genes. This study laid the foundation for the further study on the role of polygalacturonase in S. sisali pathogenicity during pathogenicity.