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杂交瘤技术自创立以来尽管得到了迅速发展,仍然存在着一些有待解决的问题。为了能对它有一个全面正确的估价,本文对杂交瘤的培育过程及其存在问题作如下介绍。用指定抗原先后两次免疫小鼠,2—4日后取其脾脏制成细胞悬液,与能连续增殖的骨髓瘤培养细胞混匀,再加入聚乙二醇以促使细胞膜融合,然后进行培养。脾细胞与骨髓瘤细胞形成一个成活的杂交瘤细胞的成功率很低,在2×10~5个脾细胞中只有一个。要找到杂交瘤细胞,有点象大海捞针。因此,必须利用选择性培养基,将那些未能融
Although the rapid development of hybridoma technology since its establishment, there are still some problems to be solved. In order to have a comprehensive and correct valuation of it, this article describes the breeding process of hybridoma and its existing problems as follows. Mice were immunized twice with the given antigen, and the spleen was taken after 2-4 days to prepare a cell suspension. The cells were cultured with myeloma cells capable of continuous proliferation and then added with polyethylene glycol to promote cell membrane fusion and then cultured. The success rate of forming a viable hybridoma between spleen cells and myeloma cells is very low, with only one of 2 × 10 ~ 5 splenocytes. To find hybridoma cells, a bit like a needle in a haystack. Therefore, it is essential to use selective media that will not melt those