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目的研究乳糖凝集素-3在肾母细胞瘤(WT)发生中的作用。方法取18例WT患儿的肿瘤组织样品,按术前是否进行化疗将其分为术前化疗WT组和术前未化疗WT组。取3例WT患儿具有正常肾功能肿瘤旁肾组织为对照组。提取各组肾组织总蛋白,Westernblot检测乳糖凝集素-3表达水平。结果乳糖凝集素-3在对照组中的表达最弱;在术前未化疗WT组表达最强,且随着病期的进展,其表达水平不断升高;术前化疗WT组乳糖凝集素-3水平显著低于术前未化疗WT组,且术前化疗时间愈长,乳糖凝集素-3水平愈低。结论乳糖凝集素-3在WT发生中有重要作用,且化疗能够抑制乳糖凝集素-3的表达。
Objective To investigate the role of Lectin-3 in the development of nephroblastoma (WT). Methods Tumor samples from 18 WT patients were divided into two groups: preoperative chemotherapy WT group and preoperative non-chemotherapy WT group. Take 3 cases of WT children with normal renal function adjacent renal tissue as control group. The total protein in kidney was extracted and the expression of Lectin-3 was detected by Western blot. Results The expression of Lectin-3 was the weakest in the control group. The expression of Lectin-3 was the highest in the untreated WT group, and the expression level of Lectin- 3 levels were significantly lower than preoperative non-chemotherapy WT group, and the longer the preoperative chemotherapy, the lower the level of lactagin-3. Conclusion Lectin-3 plays an important role in the development of WT, and chemotherapy can inhibit the expression of Lectin-3.