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目的:探讨三氧化二砷(As2O3)对人卵巢癌细胞系SKOV3细胞侵袭转移能力及其对尿激酶型纤溶酶原激活物(uPA)及尿激酶受体(uPAR)表达的影响。方法:采用0.5μmol/L、1μmol/L、2μmol/L 3种浓度的As2O3处理人卵巢癌SKOV3细胞,48h后收集细胞,采用Transwell检测细胞的侵袭转移能力,实时定量PCR及免疫细胞化学方法检测uPA、uPAR mRNA及蛋白表达的变化。结果:经不同浓度As2O3处理的细胞,穿过模拟基底膜的数目逐步减少,As2O3明显抑制SKOV3细胞的侵袭转移能力(P<0.05);细胞uPA及uPARmRNA及蛋白表达水平与对照组相比显著降低(P<0.05),其表达水平随着药物浓度的增加而降低。结论:As2O3可抑制卵巢癌细胞的侵袭转移能力,其机制可能与抑制uPA、uPAR的表达有关。
Objective: To investigate the invasion and metastasis of human ovarian cancer cell line SKOV3 and its effect on the expressions of urokinase-type plasminogen activator (uPA) and urokinase receptor (uPAR). Methods: Human ovarian cancer cell line SKOV3 was treated with 0.5μmol / L, 1μmol / L and 2μmol / L As2O3. Cells were harvested 48 hours later. The invasion and metastasis of cells were detected by real-time quantitative PCR and immunocytochemistry Changes of uPA, uPAR mRNA and protein expression. RESULTS: The number of cells treated with As2O3 at different concentrations gradually decreased and the number of cells invaded by As2O3 significantly decreased (P <0.05). The expression levels of uPA and uPAR mRNA and protein in SKOV3 cells were significantly decreased compared with the control group (P <0.05), and its expression level decreased with the increase of drug concentration. Conclusion: As2O3 can inhibit the invasion and metastasis of ovarian cancer cells, the mechanism may be related to the inhibition of uPA and uPAR expression.