以0.45μm混合纤维素酯膜为支载膜,丙烯酰胺为功能单体,N,N’-亚甲基双丙烯酰胺为交联剂,通过表面印迹法制备得到牛血清白蛋白分子印迹膜.实验对制备分子印迹膜所用模板分子、功能单体和交联剂用量进行探讨,并对目标分子识别渗透的条件进行了优化.结果表明,较非印迹膜而言,该分子印迹膜表现出良好的识别渗透能力. Using 0.45μm mixed cellulose ester film as support film, acrylamide as functional monomer and N, N’-methylenebisacrylamide as crosslinking agent, Bovine serum albumin molecularly imprinted membrane was prepared by surface blotting. Experiments on the preparation of molecularly imprinted membrane template molecules, functional monomers and cross-linking agent used to explore, and the target molecules to identify the conditions of penetration is optimized.The results show that, compared to the non-imprinted membrane, the molecularly imprinted membrane showed good The ability to identify penetration.