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目的探讨血管内皮生长因子(VEGF)及成纤维细胞生长因子3(FGF3)在甲状腺良、恶性病变组织中的表达及其表达与甲状腺癌临床病理特征的关系。方法采用免疫组织化学S-P和SABC法检测61例甲状腺癌、43例甲状腺良性病变和19例癌旁组织标本的VEGF及FGF3的表达。结果甲状腺癌、甲状腺良性病变和癌旁组织VEGF阳性表达率分别为86.9%、16.3%、10.5%,FGF3阳性表达率分别为60.7%、7.0%及5.3%的,甲状腺癌与甲状腺良性病变和癌旁组织比较,VEGF及FGF3的阳性表达率均有显著性差异(P<0.05)。VEGF在甲状腺癌T3/T4期组、预后不良组及复发组的强阳性表达率均较对照组高(P<0.05),VEGF染色分数与甲状腺癌预后指数呈正相关(r=0.323,P=0.011);FGF3的表达与甲状腺癌组织学类型、肿瘤分期等临床病理参数无相关(P>0.05),但在VEGF阳性的甲状腺癌中有更高的表达(P<0.05)。结论VEGF及FGF3的过表达可作为协助甲状腺癌诊断的一个指标,两者在甲状腺癌中有协同表达;VEGF是甲状腺癌的预后预示因子。
Objective To investigate the expression of vascular endothelial growth factor (VEGF) and fibroblast growth factor-3 (FGF3) in benign and malignant thyroid lesions and their relationship with the clinicopathological features of thyroid carcinoma. Methods The expressions of VEGF and FGF3 in 61 thyroid carcinomas, 43 thyroid benign lesions and 19 paracancerous tissues were detected by immunohistochemical S-P and SABC methods. Results The positive rate of VEGF in thyroid cancer, thyroid benign lesion and adjacent non-cancerous tissue was 86.9%, 16.3% and 10.5%, respectively. The positive rates of FGF3 expression were 60.7%, 7.0% and 5.3%, respectively. Thyroid and thyroid benign lesions and carcinomas There was a significant difference in the positive expression rates of VEGF and FGF3 between adjacent tissues (P <0.05). The strong positive expression rate of VEGF in thyroid cancer T3 / T4 stage, poor prognosis group and recurrence group was higher than that in control group (P <0.05), VEGF staining score was positively correlated with the prognosis index of thyroid carcinoma (r = 0.323, P = 0.011 ). There was no correlation between the expression of FGF3 and clinicopathological parameters (P> 0.05), but higher in VEGF-positive thyroid cancer (P <0.05). Conclusion The overexpression of VEGF and FGF3 may serve as an index to assist in the diagnosis of thyroid cancer. Both of them have synergistic expression in thyroid cancer. VEGF is the prognostic factor of thyroid cancer.