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目的:观察调心方有效部位TX0201对Aβ1-40双侧杏仁核注射所致的类阿尔茨海默病(AD)模型大鼠脑内APP mRNA、Caspase-3表达的影响。方法:应用β淀粉样蛋白1-40片段(Aβ1-40)注射入大鼠双侧杏仁核建立类AD的动物模型。实验设正常对照组、类AD模型组、TX0201组、调心方组、安理申组,均采用灌胃给药20 d;分别用RT-PCR法、免疫组化法检测大鼠脑部皮层和海马的APP mRNA、Caspase-3表达。结果:TX0201和调心方均能明显降低大鼠皮层、海马APP(MPI)mRNA和APP(KPI)mRNA的表达。模型大鼠大脑海马CA2区和皮层Caspase-3蛋白阳性细胞表达明显增多,调心方和TX0201均能明显调低Caspase-3蛋白阳性细胞的表达。结论:TX0201可能通过降低皮层、海马部位APP mRNA的表达,下调Caspase-3,减轻神经细胞凋亡,从而发挥保护神经元作用。
Objective: To observe the effect of TX0201, an effective part of Tiaoxin Recipe, on the expression of APP mRNA and Caspase-3 in the brain of Alzheimer-like (AD) model rats induced by Aβ1-40 bilateral amygdaloid injection. Methods: Aβ-40 fragment (Aβ1-40) was injected into bilateral amygdala rats to establish an AD-like animal model. In the experiment, normal control group, AD model group, TX0201 group, Tiaoxin group and Anyishen group were given intragastrically for 20 days. RT-PCR and immunohistochemistry were used to detect the expression of cortisol Hippocampal APP mRNA and Caspase-3 expression. Results: Both TX0201 and Xinxin Fang could significantly decrease the expression of APP (MPI) mRNA and APP (KPI) mRNA in rat cortex and hippocampus. The expression of Caspase-3 positive cells in hippocampal CA2 region and cortex of rats in model group increased obviously. Both TXL and TX0201 could significantly decrease the expression of Caspase-3 positive cells. Conclusion: TX0201 may protect neurons by decreasing the expression of APP mRNA in the cortex and hippocampus, down-regulating Caspase-3 and decreasing the apoptosis of nerve cells.