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目的分析原发性小血管炎患者中,中性粒细胞质抗体(抗-ANCA)、心磷脂抗体(抗-ACA)、髓过氧化物酶抗体(抗-MPO)、蛋白酶3抗体(抗-PR3)的结果,探讨其在原发性小血管炎患者中检测的临床价值。方法收集54例送该中心诊断为原发性小血管炎患者的血清和20例健康对照组血清,采用间接免疫荧光法使用德国欧蒙试剂检测抗-ANCA,采用酶联免疫吸附法使用德国AESKU试剂检测抗-ACA,采用免疫印迹法使用德国欧蒙试剂检测抗-MPO和抗-PR3。结果在54例原发性小血管炎患者中,抗-ANCA的阳性率为50%,抗-ACA的阳性率为52%,抗-MPO的阳性率为11%,抗-PR3的阳性率为9.26%,抗-AN-CA与抗-ACA联合检测的阳性率为100%,抗-ANCA与抗-MPO、抗-PR3联合检测的阳性率为50%。结论抗-ANCA、抗-MPO、抗-PR3在原发性小血管炎患者中具有重要的检测意义,其与抗-ACA联合检测,能提高原发性小血管炎的诊断敏感性。
Objective To analyze the relationship between neutrophil cytoplasmic antibody (anti-ANCA), cardiolipin antibody (anti-ACA), myeloperoxidase antibody (anti-MPO), protease 3 antibody ), To explore its clinical value in the detection of patients with primary small vessel vasculitis. Methods Serum samples of 54 patients with primary small vessel vasculitis and 20 healthy controls were collected and detected by indirect immunofluorescence using anti-ANCA in Germany. Enzyme-linked immunosorbent assay Reagents were tested for anti-ACA, and Western blotting was used to detect anti-MPO and anti-PR3 using German Aumon Reagent. Results In 54 cases of primary small vessel vasculitis, the positive rate of anti-ANCA was 50%, the positive rate of anti-ACA was 52%, the positive rate of anti-MPO was 11%, the positive rate of anti-PR3 was 9.26%. The positive rate of anti-AN-CA combined with anti-ACA was 100%. The positive rate of anti-ANCA combined with anti-MPO and anti-PR3 was 50%. CONCLUSION: Anti-ANCA, anti-MPO and anti-PR3 have important significance in the diagnosis of patients with primary small vessel vasculitis. Combined with anti-ACA, they can improve the diagnostic sensitivity of primary small vessel vasculitis.