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目的对2013年云南省22株柯萨奇病毒A6型(coxsackievirus A6,CV-A6)VP1区基因特征进行分析。方法对云南省2013年手足口病实验室监测系统分离到的22株CV-A6病毒VP1区基因进行基因扩增和测序;按参考文献下载基因库中CV-A6病毒VP1区基因参考序列,用MEGA 5.0软件计算不同毒株的核苷酸和氨基酸差异率,并构建系统进化树,进行基因特征和分子流行病学分析。结果 2013年云南省在711份非肠道病毒71型(EV-A71)、非柯萨奇病毒A16型(CV-A16)肠道病毒中共检出CV-A6病毒22份,占3.09%(22/711),CV-A6的阳性总检出率为0.32%(22/6 912)。基因特征分析表明,云南22株CV-A6均为基因1型(Genotype 1)。它们分为3个不同的进化分支,1株为China cluster,6株为Yunnan cluster 1,15株为Yunnan cluster 2。结论除原型株Gdula(AF081297)外,国内外CV-A6病毒存在两个基因型,基因型2又分为8个进化分支,云南分离株分布在3个不同的进化分支,2013年云南省CV-A6的流行存在3个传播链。
Objective To analyze the characteristics of VP1 gene of 22 Coxsackievirus A6 (CV-A6) strains in Yunnan Province in 2013. Methods 22 strains of VP1 gene of CV-A6 virus isolated from laboratory of HFMD in Yunnan Province in 2013 were amplified and sequenced. The reference sequence of VP1 gene of CV-A6 virus in gene bank was downloaded from the reference database, The MEGA 5.0 software calculates the nucleotide and amino acid difference of different strains and constructs the phylogenetic tree for gene characterization and molecular epidemiological analysis. Results In 2013, 22 CV-A6 viruses were detected in 711 enterovirus 71 (EV-A71) and non-Coxsackie A16 (CV-A16) enterovirus in Yunnan Province, accounting for 3.09% (22 / 711). The overall positive detection rate of CV-A6 was 0.32% (22/6 912). Gene analysis showed that 22 strains of CV-A6 were genotype 1 in Yunnan. They are divided into three different evolutionary branches, one for China cluster, six for Yunnan cluster 1 and 15 for Yunnan cluster 2. Conclusion In addition to the prototype strain Gdula (AF081297), there are two genotypes of CV-A6 virus at home and abroad, genotype 2 is divided into eight evolutionary branches, Yunnan isolates are distributed in three different evolutionary branches. In 2013, Yunnan CV The popularity of -A6 There are three transmission chains.