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目的探讨人类白细胞抗原(Human Leukocyte Antigens,HLA)DP基因多态性与高危人群丙型肝炎病毒(Hepatitis C Virus,HCV)感染转归的关系。方法采用病例对照研究设计,运用Taqman-MGB技术检测401例HCV持续感染者,268例HCV自限感染者和984例健康对照者HLA-DP基因rs3077、rs9277535、rs2395309位点的基因多态性,比较不同基因型与HCV感染转归的关系。结果采用Logistic回归分析调整性别、年龄和高危人群种类混杂因素后结果显示,rs3077位点:杂合基因型CT和突变型TT在感染组(持续感染组+自限清除组)中的频率均显著高于对照组(调整OR值及其95%CI分别为1.31,1.05~1.64和1.93,1.36~2.73);rs2395309位点:杂合基因型GA和突变型AA在感染组中的频率均显著高于对照组(调整OR值及其95%CI分别为1.29,1.03~1.61和1.89,1.34~2.68);rs9277535位点:杂合基因型GA和突变型AA在持续性感染组中的频率均高于自限清除组(P=0.018,P=0.037)。结论rs3077、rs9277535、rs2395309三个位点的基因多态性可能与丙型肝炎转归有一定的关联。
Objective To investigate the association between DP gene polymorphism of Human Leukocyte Antigens (HLA) and the prognosis of high-risk population of Hepatitis C Virus (HCV) infection. Methods A case-control study was designed. The gene polymorphisms of rs3077, rs9277535 and rs2395309 of HLA-DP gene in 401 cases of persistent HCV infection, 268 cases of HCV self-limited infection and 984 healthy controls were detected by Taqman-MGB technique. Compare the relationship between different genotypes and the outcome of HCV infection. Results Using logistic regression analysis to adjust the gender, age and high risk population confounding factors, the results showed that rs3077 locus: heterozygous genotype CT and mutant TT in the infected group (persistent infection group + self-limited clearance group) were significantly (Adjusted odds ratio and its 95% CI were 1.31, 1.05 ~ 1.64 and 1.93, 1.36 ~ 2.73, respectively). At rs2395309 locus, the frequencies of heterozygous GA and mutant AA in the infected group were significantly higher than those in the control group (OR = 95% CI, 1.29, 1.03-1.61 and 1.89, 1.34-2.68, respectively) in the control group. The frequency of rs9277535 locus: heterozygous GA and mutant AA in the persistent infection group At self-limited clearance group (P = 0.018, P = 0.037). Conclusion The polymorphisms of rs3077, rs9277535 and rs2395309 may be related to the prognosis of hepatitis C patients.