论文部分内容阅读
人工合成的10个不同血吸虫多肽片段对小鼠进行免疫保护实验，采用200μg／只皮下注射，共免疫3次，每次间隔1周，末次免疫后2周，以（40±2）条日本血吸虫正常尾蚴攻击感染。另设不免疫攻击感染日本血吸虫的小鼠为对照组。实验组小鼠分别获得10．7％～73．6％的减虫率、23．0％～75．9％的减卵率和－11．22％～66．78％的虫卵结节减小率。各实验组血清抗体均为阳性，随时间的延长有增高趋势。实验提示，以寡赖氨酸为母校，连接4个或8个抗原肽的多肽抗原效果比较理想。
Immunized mice were immunized with 10 different schistosome peptide fragments. The mice were immunized with 200 μg / subcutaneously three times at intervals of 1 week. Two weeks after the last immunization, the mice were immunized with (40 ± 2) Normal cercariae attack infection. Another non-immune attack in mice infected with Schistosoma japonicum as a control group. The mice in the experimental group got the worm reduction rates of 10.7% -73.6%, the egg reduction rates of 23.0% -75.9% and the egg-derived nodules of -11.22% -66.78% respectively Small rate. Serum antibodies in each experimental group were positive, with the extension of time increasing trend. Experimental results suggest that the use of oligo-lysine as an alma mater, connecting four or eight peptide antigen peptide antigen ideal results.