介绍草乌毒蛋白的提取方法。草乌根经pH7.2的PBS溶液 (含9g/LNaCl)浸提,浸提液经CM -SFF柱和SephacrylS -200凝胶过滤柱分离,然后在高效凝胶过滤柱上制备ACO毒蛋白,利用光电二极管阵列检测器的光谱性能确认色谱峰的纯度,并根据标准蛋白的相对分子质量校正曲线求得ACO的相对分子质量,用柱后衍生荧光法测定了其氨基酸组成 Introduce the method of extracting the crude protein of toxin. The crude extracts were extracted with PBS (containing 9 g / L NaCl) at pH 7.2. The extract was separated on a CM-SFF column and a Sephacryl S-200 gel filtration column. ACO toxin was prepared on a high performance gel filtration column. The purity of the chromatographic peak was confirmed by the spectral performance of the photodiode array detector. The relative molecular mass of ACO was determined according to the calibration curve of the relative molecular mass of the standard protein. The amino acid composition was determined by post-column derivatization