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对荔枝幼胚体细胞胚胎发生的影响因素进行研究。实验表明，来源于３０天龄幼胚的愈伤组织容易诱导出胚性愈伤组织。在８．０ｍｇ·Ｌ－１２，４Ｄ＋０．２ｍｇ·Ｌ－１ＮＡＡ和５％蔗糖的ＭＳ培养基上，作为期７～１０天短暂的激发培养后，胚性愈伤组织的诱导率可达６４．２％。在含１．０ｍｇ·Ｌ－１２，４Ｄ的培养基上继代增殖并保持胚性。在去掉２，４Ｄ，降低蔗糖浓度为３％，附加０．２ｍｇ·Ｌ－１ＮＡＡ＋０．１ｍｇ·Ｌ－１ＩＢＡ的液体培养基上振荡培养，形成体胚的胚性愈伤组织频率为６２％。培养成的正常成熟胚在１／２ＭＳ附加０．２ｍｇ·Ｌ－１ＮＡＡ＋１．０ｍｇ·Ｌ－１ＩＢＡ和２％蔗糖的固体培养基上萌发成苗率为２２．３％。
The influencing factors of somatic embryogenesis in litchi embryos were studied. Experiments show that callus from 30-day-old immature embryos can easily induce embryogenic callus. The induction rate of embryogenic callus on MS medium 8.0mg · L-12,4D + 0.2mg · L-1NAA and 5% sucrose after a short period of 7 ~ 10 days excitation culture 64.2%. In 1.0mg · L-12,4 D medium on the proliferation and maintain embryogenic. In the removal of 2,4 D, reducing the concentration of sucrose 3%, supplemented with 0.2mg · L-1NAA + 0.1mg · L-1IBA liquid medium shaking culture embryogenic callus embryogenic frequency of 62% . The normal mature embryos were cultured on 1 / 2MS solid medium supplemented with 0.2 mg · L-1 NAA + 1.0 mg · L-1 IBA and 2% sucrose and the seedling rate was 22.3%.