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作者为了寻找杀灭配子体的药物能有逻辑依据,以不同代谢抑制剂对体外培养的恶性疟原虫配子体的发育影响作了实验观察,虫源取自冈比亚自然感染的恶性疟患者。采新鲜血液培养作筛选试验,同时进行液氮冻存。19人中筛出6人血中可产生配子体,以此6人的冻存标本复苏后培养进行药物试验。采用 Smalley(1976及1977) 的微量培养法和药物筛选方法。所用药物有放线菌素D、8-氮鸟嘌呤、秋水仙碱、放线菌酮、盐酸吐根素、丝裂霉素 C、二盐酸嘌呤霉素和利福平。观察期为9天,每2天更换培养液1次。原虫与药物接触时间为48小时。配子体的形态发育以 Hawking 等(1971) 所划分的5个阶段为标准,在观察药物对配子体各期作用的同时,对无性体的影响也作了观察。
In order to search for drugs to kill gametophytes, the author can make a logical observation based on the experimental observation of the developmental effects of different metabolic inhibitors on P. falciparum gametocytes cultured in vitro. The insect sources were obtained from naturally infected P. falciparum in the Gambia. Fresh blood culture was used for screening tests, while liquid nitrogen frozen. 19 out of 6 people in the blood can produce gametophyte, so that 6 frozen specimens after the recovery of cultured drug test. Smalley (1976 and 1977) of the micro-culture and drug screening methods. The drugs used were actinomycin D, 8-azaguanine, colchicine, cycloheximide, ibuprofen hydrochloride, mitomycin C, puromycin dihydrochloride and rifampicin. The observation period was 9 days and the culture medium was changed every 2 days. Protozoa and drug contact time is 48 hours. The morphological development of gametophytes is based on the five stages divided by Hawking et al. (1971). While observing the effect of the drugs on the gametophytic stages, the effects on the vegetative organs were also observed.